Klebsiella Pneumoniae, Acinetobacter Baumannii and Pseudomonas Aeruginosa and Drug Resistance Genes (KPC, NDM, OXA48 and IMP) Multiplex
Product name
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii and Pseudomonas Aeruginosa and Drug Resistance Genes (KPC, NDM, OXA48 and IMP) Multiplex Detection Kit(Fluorescence PCR)
Certificate
CE
Epidemiology
Klebsiella pneumoniae is a common clinical opportunistic pathogen and one of the important pathogenic bacteria causing nosocomial infections. When the body's resistance is reduced, the bacteria enter the lungs from the respiratory tract, causing infection in multiple parts of the body, and the early use of antibiotics is the key to cure [1].
The most common site of Acinetobacter baumannii infection is the lungs, which is an important pathogen for Hospital acquired pneumonia (HAP), especially Ventilator associated pneumonia (VAP). It is often accompanied by other bacterial and fungal infections, with characteristics of high morbidity rate and high mortality rate.
Pseudomonas aeruginosa is the most common non-fermentative gram-negative bacilli in clinical practice, and is an important opportunistic pathogen for hospital-acquired infection, with the characteristics of easy colonization, easy variation and multi-drug resistance.
Channel
| Name | PCR-Mix 1 | PCR-Mix 2 |
| FAM Channel | Aba | IMP |
| VIC/HEX Channel | Internal Control | KPC |
| CY5 Channel | PA | NDM |
| ROX Channel | KPN | OXA48 |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Sputum |
| Ct | ≤36 |
| CV | ≤10.0% |
| LoD | 1000 CFU/mL |
| Specificity | a)The cross-reactivity test shows that this kit has no cross reactivity with other respiratory pathogens, such as Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus and sputum samples without targets, etc.
b)Anti-interference ability: Select mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, and roxithromycin, etc. for the interference test, and the results show that the interference substances mentioned above do not interfere with the detection of Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and carbapenem resistance genes KPC, NDM, OXA48 and IMP. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Total PCR Solution
