Human CYP2C9 and VKORC1 Gene Polymorphism

Short Description:

This kit is applicable to in vitro qualitative detection of polymorphism of CYP2C9*3 (rs1057910, 1075A>C) and VKORC1 (rs9923231, -1639G>A) in the genomic DNA of human whole blood samples.

Product Detail

Product Tags

Product name

HWTS-GE014A-Human CYP2C9 and VKORC1 Gene Polymorphism Detection Kit (Fluorescence PCR)




Warfarin is an oral anticoagulant commonly used in clinical practice currently, which is mainly intended for the prevention and treatment of thromboembolic diseases. However, warfarin has a limited treatment window and varies greatly among different races and individuals. Statistics have indicated that the difference of stable dose in different individuals can be more than 20 times. The adverse reaction bleeding occurs in 15.2% of the patients taking warfarin each year, of which 3.5% develop fatal bleeding. Pharmacogenomic studies have shown that genetic polymorphism of target enzyme VKORC1 and metabolic enzyme CYP2C9 of warfarin is an important factor affecting the difference in the dose of warfarin. Warfarin is a specific inhibitor of vitamin K epoxide reductase (VKORC1), and thus inhibits clotting factor synthesis involving vitamin K and provides anticoagulation. A large number of studies have indicated that the gene polymorphism of VKORC1 promoter is the most important factor affecting the race and individual differences in the required dose of warfarin. Warfarin is metabolized by CYP2C9, and its mutants greatly slower metabolism of warfarin. Individuals using warfarin have a higher risk (twice to three times higher) of bleeding in the early stage of use. 


FAM VKORC1 (-1639G>A)
CY5 CYP2C9*3

Technical Parameters

Storage Liquid: ≤-18℃
Shelf-life 12 months
Specimen Type Fresh EDTA anticoagulated blood
CV ≤5.0%
LoD 1.0ng/μL
Specificity There is no cross-reactivity with other highly consistent sequence of human genome (human CYP2C19 gene, human RPN2 gene); mutation of CYP2C9*13 and VKORC1 (3730G>A) outside the detection range of this kit
Applicable Instruments Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006). 

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