Influenza A Virus Universal/H1/H3
Product name
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit(Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) is the smallest prokaryotic microorganism with a cell structure and no cell wall between bacteria and viruses. MP mainly causes respiratory tract infections in humans, especially in children and young people. MP can cause Mycoplasma hominis pneumonia, respiratory tract infections in children and atypical pneumonia. The clinical symptoms are diverse, mostly severe cough, fever, chills, headache, sore throat, upper respiratory tract infection and bronchopneumonia are the most common. Some patients may develop severe pneumonia from upper respiratory tract infection, and severe respiratory distress or even death may occur. MP is one of the common and important pathogens in community-acquired pneumonia (CAP), accounting for 10%-30% of CAP, and the proportion can increase 3-5 times when MP is prevalent. In recent years, the proportion of MP in CAP pathogens has gradually increased. The incidence of Mycoplasma pneumoniae infection has increased, and because of its nonspecific clinical manifestations, it is easy to be confused with bacterial and viral colds. Therefore, early laboratory detection is of great significance for clinical diagnosis and treatment.
Channel
FAM | influenza A universal type virus nucleic acid |
VIC/HEX | influenza A H1 type virus nucleic acid |
ROX | influenza A H3 type virus nucleic acid |
CY5 | internal control |
Technical Parameters
Storage |
≤-18℃ |
Shelf-life | 9 months |
Specimen Type | nasopharyngeal swab |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 500 Copies/μL |
Specificity |
No cross-reactivity with other respiratory samples such as Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. and human genomic DNA. |
Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System
Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Nucleic Acid Extraction or Purification Reagent (YDP315-R) by Tiangen Biotech (Beijing) Co.,Ltd. The extraction should be carried out strictly according to the instructions for use. The extracted sample volume is 140μL, and the recommended elution volume is 60μL.
Option 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). The extraction should be carried out strictly according to the instructions for use. The extracted sample volume is 200μL, and the recommended elution volume is 80μL.