STI Multiplex 7 Detection Kit

Short Description:

Product Code

HWTS-UR012

Intended Use

This kit is intended for qualitative detection of common pathogens of urogenital infections, including Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU), Herpes Simplex Virus Type 1 (HSV1), Herpes Simplex Virus Type 2 (HSV2), Mycoplasma hominis (Mh), Mycoplasma genitalium ( Mg) in male urinary tract and female genital tract secretion samples.

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Product Detail

Product Tags

Product Name

STI Multiplex Detection Kit

Epidemiology

Sexually transmitted infection (STI) remain one of the major threats to global public health security, which can lead to infertility, preterm birth, tumorigenesis, and various serious complications. There are many types of STI pathogens, including bacteria, viruses, chlamydia, mycoplasma, and spirochetes. NG, CT, UU, HSV 1, HSV 2, Mh, Mg are more common.

Technical Parameters

Storage	≤ -18℃
Shelf-life	12 months
Specimen Type	Urethral secretions, Cervical secretions
CV	<5.0%
LoD	Limit of Detection (LoD): The LoD of the kit for detecting CT, NG, UU, HSV1, HSV2 and Mg is 400Copies/mL; the LoD of the kit for detecting Mh is 1000Copies/mL.
Specificity	There is no cross reaction with similar STI pathogens.
Applicable Instruments	Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR systems, SLAN® -96P Real-Time PCR Systems, LightCycler® 480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) , BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR Systemand and etc.

Channel

Reaction Buffer	Target	Reporter	Quencher
STI Reaction Buffer 1	CT	FAM	none
	UU	VIC（HEX）	none
	Mh	ROX	none
	HSV1	CY5	none
STI Reaction Buffer 2	NG	FAM	none
	HSV2	VIC（HEX）	none
	Mg	ROX	none
	IC	CY5	none

Work Flow

Reagents need but not provided

Macro & Micro-Test Sample Release Reagent (HWTS-3005-8); Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The extraction should be conducted according to the IFU.

Consumables need but not provided: 1.5mL of DNase/RNase free centrifuge tube, DNase/RNase free Tips, desktop entrifuge, desktop oscillation mixer.

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Chlamydia trachomatis (CT)