Chlamydia Trachomatis Nucleic Acid Detection Kit (Fluorescence PCR)

Short Description:

This kit is used for qualitative detection of Chlamydia trachomatis nucleic acid in male urine, male urethral swab, and female cervical swab samples.

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Product Detail

Product Tags

Product Name

Chlamydia Trachomatis Nucleic Acid Detection Kit (Fluorescence PCR)

Intended Use

This kit is used for qualitative detection of Chlamydia trachomatis nucleic acid in male urine, male urethral swab, and female cervical swab samples.

Epidemiology

Chlamydia trachomatis (CT) is a kind of prokaryotic microorganism that is strictly parasitic in eukaryotic cells. Chlamydia trachomatis is divided into A-K serotypes according to the serotype method. Urogenital tract infections are mostly caused by trachoma biological variant D-K serotypes, and males are mostly manifested as urethritis, which can be relieved without treatment, but most of them become chronic, periodically aggravated, and can be combined with epididymitis, proctitis, etc. Females can be caused with urethritis, cervicitis, etc., and more serious complications of salpingitis.

Epidemiology

FAM: Chlamydia trachomatis (CT)·

VIC(HEX): Internal Control

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	5mins	No
2	1 cycle	95℃	10mins	No
3	40 cycles	95℃	15 secs	No
4	40 cycles	58℃	31 secs	Yes

Technical Parameters

Storage	≤-18℃ In dark
Shelf-life	12 months
Specimen Type	Male urethral secretions, Female cervical secretions, Male urine
Ct	≤38
CV	＜5.0%
LoD	400Copies/mL
Specificity	There is no cross-reactivity for detecting other STD-infected pathogens by this kit, such as Treponema pallidum, Neisseria gonorrhoeae, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, etc., which are outside the detection range of kit.
Applicable Instruments	It can match the mainstream fluorescent PCR instruments on the market. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio^® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler^®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System

Workflow

Chlamydia Trachomatis Nucleic Acid Detection Kit (Fluorescence PCR)

Reagents need but not provided

Normal saline, Macro & Micro-Test Sample Collecting, Mailing & Shipping Kit (HWTS-3030-1B), Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), Nucleic Acid Extraction or Purification Reagent (YDP302) by Tiangen Biotech (Beijing) Co., Ltd.