Staphylococcus Aureus and Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Product name
HWTS-OT062 Staphylococcus Aureus and Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit(Fluorescence PCR)
Certificate
CE
Epidemiology
Staphylococcus aureus is one of the important pathogenic bacteria of nosocomial infection. Staphylococcus aureus (SA) belongs to the staphylococcus and is a representative of Gram-positive bacteria, which can produce a variety of toxins and invasive enzymes. The bacteria have the characteristics of wide distribution, strong pathogenicity and high resistance rate. Thermostable nuclease gene (nuc) is a highly conserved gene of staphylococcus aureus.
Channel
| FAM | methicillin-resistant mecA gene |
| ROX |
Internal Control |
| CY5 | staphylococcus aureus nuc gene |
Technical Parameters
| Storage | ≤-18℃ & protected from light |
| Shelf-life | 12 months |
| Specimen Type | sputum, skin and soft tissue infection samples, and nasal swab samples |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL methicillin-resistant bacteria. When the kit detects the national LoD reference, 1000/mL staphylococcus aureus can be detected |
| Specificity | The cross-reactivity test shows that this kit has no cross reactivity with other other respiratory pathogens such as methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Add 200µL of normal saline to the processed precipitate, and the subsequent steps should be extracted according to the instructions, and the recommended elution volume is 80µL.
Option 2.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Add 1mL of normal saline to the precipitate after washing with normal saline, then mix well. Centrifuge at 13,000r/min for 5 minutes, remove the supernatant (reserve 10-20µL of supernatant), and follow the instructions for subsequent extraction.
Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent (YDP302) by Tiangen Biotech (Beijing) Co.,Ltd. The extraction should be carried out strictly according to the step 2 of the instruction manual. It is recommended to use RNase and DNase-free water for elution with the volume of 100µL.
