12 Kinds of Respiratory Pathogen
Product name
HWTS-RT071A 12 Kinds of Respiratory Pathogen Nucleic Acid Detection Kit (Fluorescence PCR)
Channel
| Channel | hu12 Reaction Buffer A | hu12 Reaction Buffer B | hu12 Reaction Buffer C | hu12 Reaction Buffer D |
| FAM | SARS-CoV-2 | HADV | HPIV Ⅰ | HRV |
| VIC/HEX | Internal Control | Internal Control | HPIV Ⅱ | Internal Control |
| CY5 | IFV A | MP | HPIV Ⅲ | / |
| ROX | IFV B | RSV | HPIV Ⅳ | HMPV |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | SARS-CoV-2：300 Copies/mLinfluenza B virus：500 Copies/mLinfluenza A virus：500 Copies/mL
Adenovirus：500 Copies/mL mycoplasma pneumoniae：500 Copies/mL respiratory syncytial virus：500 Copies/mL, parainfluenza virus (Ⅰ, Ⅱ, Ⅲ, Ⅳ)：500 Copies/mL Rhinovirus：500 Copies/mL human metapneumovirus：500 Copies/mL |
| Specificity | The cross-reactivity study shows that there is no cross-reactivity between this kit and enterovirus A, B, C, D, epstein-barr virus, measles virus, human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella-herpes zoster virus, bordetella pertussis, streptococcus pyogenes, mycobacterium tuberculosis, aspergillus fumigatus, candida albicans, candida glabrata, pneumocystis jirovecii, cryptococcus neoformans and human genomic nucleic acid. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7500 Fast Real-Time PCR SystemsQuantStudio®5 Real-Time PCR Systems
SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd. ) LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection System（FQD-96A，Hangzhou Bioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
Recommended extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., The extraction should be carried out in strict accordance with the instructions.
Option 2.
Recommended extraction reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., the extraction should be conducted according to the instructions strictly. The recommended elution volume is 80μL.
Option 3.
Recommended extraction reagent: Nucleic Acid Extraction or Purification Kit (YDP315) by Tiangen Biotech(Beijing) Co.,Ltd., the extraction should be conducted according to the instructions strictly. The recommended elution volume is 100μL.
