Real-time fluorescent RT-PCR kit for detecting SARS-CoV-2
Product name
HWTS-RT057A-Real-time fluorescent RT-PCR kit for detecting SARS-CoV-2
HWTS-RT057F-Freeze-dried Real-time fluorescent RT-PCR kit for detecting SARS-CoV-2 -Subpackage
Certificate
CE
Epidemiology
The novel coronavirus (SARS-CoV-2) has spread on a large scale around the world. In the process of dissemination, new mutations constantly occur, resulting in new variants. This product is mainly used for the auxiliary detection and differentiation of cases related to infection after the large-scale spread of Alpha, Beta, Gamma, Delta and Omicron mutant strains since December 2020.
Channel
| FAM | 2019-nCoV ORF1ab gene |
| CY5 | 2019-nCoV N gene |
| VIC(HEX) | internal reference gene |
Technical Parameters
|
Storage |
Liquid: ≤-18℃ In dark |
|
Lyophilized: ≤30℃ In dark |
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|
Shelf-life |
Liquid: 9 months |
|
Lyophilized: 12 months |
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Specimen Type |
nasopharyngeal swabs, oropharyngeal swabs |
|
CV |
≤5.0% |
|
Ct |
≤38 |
|
LoD |
300Copies/mL |
|
Specificity |
There is no cross-reactivity with human coronaviruses SARS-CoV and other common pathogens. |
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Applicable Instruments: |
Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems SLAN ®-96P Real-Time PCR Systems QuantStudio™ 5 Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
Nucleic Acid Extraction or Purification Kit（Magnetic Beads Method）(HWTS-3001, HWTS-3004-32, HWTS-3004-48) from Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Option 2.
Recommended extraction reagent: QIAamp Viral RNA Mini Kit (52904), Viral RNA Extraction Kit (YDP315-R) manufactured by Tiangen Biotech (Beijing) Co., Ltd.
