Manufactur standard Ebv Infection - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test

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Manufactur standard Ebv Infection - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

EB Virus Nucleic Acid Detection Kit(Fluorescence PCR)

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Epidemiology

EBV (Epstein-barr virus), or human herpesvirus type 4, is a common human herpesvirus^[1]. In recent years, a large number of studies have proved that EBV is associated with occurrence and development of nasopharyngeal cancer, Hodgkin’s disease, T/Natural killer celllymphoma, Burkitt’s lymphoma, breast cancer, gastric cancer and other malignant tumors. And it is also closely associated with post-transplantlymphoproliferative disorders, post-transplant smooth muscle tumor and acquired immunedeficiency syndrome(AIDS) related lymphoma, multiple sclerosis, primary central nervous system lymphoma or leiomyosarcoma^[2-4].

Features

● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 500Copies/mL.
● Convenient: One test for multiple results.

Channel

FAM: EBV
VIC (HEX): Internal control

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	2mins	No
2	1 cycle	95℃	5mins	No
3	40 cycles	95℃	15s	No
3	40 cycles	58℃	30s	Yes

Technical Parameters

Storage	≤-18℃ In dark
Shelf-life	12 months
Specimen Type	Whole blood, Plasma, Serum
Ct	≤38
CV	≤5.0％
LoD	500Copies/mL

Specificity: It has no cross-reactivity with other pathogens (such as human herpesvirus 1, 2, 3, 6, 7, 8, hepatitis B virus, cytomegalovirus, influenza A, etc.) or bacteria (Staphylococcus aureus, Candida albicans, etc.)

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.

Main Components

Catalogue Number	Component (50 tests/kit)	Specification	Quantity	Component Description
HWTS-OT061A	EB Reaction Buffer	1.175mL/vial	1 vial	Amplification reaction reagents, EB virus primer probes, internal reference primer probes, etc.
	EB Enzyme Mix	75μL/vial	1 vial	Taq enzyme, UDG enzyme, etc.
	EB Positive Control	600μL/vial	1 vial	Mixed solution of viral target gene and internal reference
	EB Blank Control	600μL/vial	1 vial	DNase/RNase free H₂O

Total PCR Solution

EB Virus Nucleic Acid Detection Kit(Fluorescence PCR)6

Reference

[1] ohen JI, Jaffe ES, Dale JK, et al. Characterization and treatment of chronic active Epstein-Barr virus disease: a 28-year experience in the United States[J]. Blood, 2011, 117(22): 5835-5849.
[2] Jahangiryan A, Kheirandish M, Samiee S, et al. Determination of Epstein-Barr virus (EBV) incidence in umbilical cord blood (UCB) and assessment of virus DNA via Real-time PCR[J]. Annals of Cancer Research and Therapy, 2021, 29(1):114-120.
[3] Petersson F. Nasopharyngeal carcinoma:a review[J].Semin Diagn Pathol, 2015, 32(1):54-73. [4]Chen J N, He D, Tang F, et al. Epstein-Barr virus-associated gastric carcinoma:a newly defined entity[J]. J Clin Gastroenterol, 2012, 46(4):262-271.

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Our products are widely recognized and trusted by users and can meet continuously developing economic and social needs for Manufactur standard Ebv Infection - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test , The product will supply to all over the world, such as: Chicago, Thailand, Ethiopia, Our products are produced with the best raw materials. Every moment, we constantly improve the production programme. In order to ensure better quality and service, we have been focusing on the production process. We have got high praise by partner. We are looking forward to establishing business relationship with you.

Eb Virus Nucleic Acid Detection Kit(Fluorescence Pcr)