Legionella Pneumophila Nucleic Acid Detection Kit

Short Description:

This kit is used for the qualitative detection of legionella pneumophila nucleic acid in sputum samples of patients with suspected legionella pneumophila infection, and provides aid to the diagnosis of patients with legionella pneumophila infection.


Product Detail

Product Tags

Product name

HWTS-RT163-Legionella Pneumophila Nucleic Acid Detection Kit(Fluorescence PCR)

Epidemiology

Legionella pneumophila is a flagellated, gram-negative, short coccobacillus of the Legionella genus polymorphic. Legionella pneumophila is a facultative parasitic bacterium that can invade amoeba or human macrophages. The infectivity of this bacterium is greatly enhanced in the presence of antibodies and serum complement (but the presence of both is not absolutely required). Legionella pneumophila is an important pathogen causing epidemic and sporadic community-acquired pneumonia and hospital-acquired pneumonia, accounting for approximately 80% of Legionella pneumonia. Legionella pneumophila mainly exists in water and soil. Absorption of contaminated water and soil into the human body in the form of aerosol may be the main route of Legionella infection. At present, the main methods for laboratory detection and diagnosis of Legionella pneumophila are bacterial culture and serological assay.

Technical Parameters

Storage -18℃
Shelf-life 12 months
Specimen Type sputum 
Ct ≤38
CV 5.0%
LoD 1000 Copies/μL
Applicable Instruments Applicable to type I detection reagent:
Applied Biosystems 7500 Real-Time PCR Systems,QuantStudio®5 Real-Time PCR Systems,SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.),LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology),MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.),BioRad CFX96 Real-Time PCR System,BioRad CFX Opus 96 Real-Time PCR System.
Applicable to type II detection reagent:
EudemonTM AIO800 (HWTS-EQ007) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

 

Work Flow

Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), and Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (which can be used with EudemonTM AIO800 (HWTS-EQ007)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

The extracted sample volume is 200μL and the recommended elution volume is 150μL.


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