Freeze-dried 11 Kinds of Respiratory Pathogens Nucleic Acid

Short Description:

This kit is used for qualitative detection of common respiratory pathogens in human sputum, including Haemophilus influenzae (HI), Streptococcus pneumoniae (SP), Acinetobacter baumannii (ABA), Pseudomonas aeruginosa (PA), Klebsiella pneumoniae (KPN), Stenotrophomonas maltophilia (Smet), Bordetella pertussis (Bp), Bacillus parapertusss (Bpp), Mycoplasma pneumoniae (MP), Chlamydia pneumoniae (Cpn), Legionella pneumophila (Leg). The test results can be used for auxiliary diagnosis of hospitalized patients or critically ill patients with suspected bacterial infection of the respiratory tract.


Product Detail

Product Tags

Product name

HWTS-RT190 -Freeze-dried-Freeze-dried 11 Kinds of Respiratory Pathogens Nucleic Acid Detection Kit(Fluorescence PCR)

Epidemiology

Respiratory tract infection is an important disease that seriously threatens humanhealth.  Studies have shown that most respiratory tract infections are caused by bacterial and/or viral pathogens that co-infect the host, leading to increased severity of the disease or even death.  Therefore, identifying the pathogen can provide targeted treatment and improve the patient's survival rate[1,2].  However, traditional methods for detecting respiratory pathogens include microscopic examination,bacterial culture, and immunological examination.  These methods are complex, time-consuming, technically demanding, and have low sensitivity.  In addition, they cannot detect multiple pathogens in a single sample, making it difficult to provide doctors with accurate auxiliary diagnosis.  As a result, most medications are still in the empirical medication stage, which not only accelerates the cycle of bacterial resistance, but also affects the timely diagnosis of patients[3].  Common Haemophilus influenzae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila are important pathogens that cause nosocomial respiratory tract infections[4,5].  This test kit detects and identifies the specific nucleic acids of the above pathogens in individuals with signs and symptoms of respiratory infection, and combines it with other laboratory results to assist in the diagnosis of respiratory pathogen infection.

Technical Parameters

Storage

2-30℃

Shelf-life 12 months
Specimen Type Throat swab
Ct ≤33
CV <5.0%
LoD The LoD of the kit for Klebsiella pneumoniae is 500 CFU/mL; the LoD of Streptococcus pneumoniae is 500 CFU/mL; the LoD of Haemophilus influenzae is 1000 CFU/mL; the LoD of Pseudomonas aeruginosa is 500 CFU/mL; the LoD of Acinetobacter baumannii is 500 CFU/mL; the LoD of Stenotrophomonas maltophilia is 1000 CFU/mL; the LoD of Bordetella pertussis is 500 CFU/mL; the LoD of Bordetella parapertussis is 500 CFU/mL; the LoD of Mycoplasma pneumoniae is 200 copies/mL; the LoD of Legionella pneumophila is 1000 CFU/mL; the LoD of Chlamydia pneumoniae is 200 copies/mL.
Specificity There is no cross reaction between the kit and other common respiratory pathogens outside the detection range of the test kit, e.g. Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoca, Streptococcus pyogenes, Micrococcus luteus, Rhodococcus equi, Listeria monocytogenes, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium tuberculosis, Influenza A virus, Influenza B virus, Aspergillus flavus, Aspergillus terreus, Aspergillus fumigatus, Candida glabrata, and Candida tropicalis.
Applicable Instruments

Type I: Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR Systems, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System.

Type II: EudemonTM AIO800 (HWTS-EQ007) by Jiangsu Macro & Micro-Test Med-Tech Co. Ltd.

Work Flow

Type I: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. are recommended for the sample extraction and the subsequent steps should be conducted in strict accordance with the IFU of the Kit. 


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