Adenovirus Type 41 Nucleic Acid

Short Description:

This kit is used for the qualitative detection of adenovirus nucleic acid in stool samples in vitro.


Product Detail

Product Tags

Product name

HWTS-RT113-Adenovirus Type 41 Nucleic Acid Detection Kit(Fluorescence PCR)

Epidemiology

Adenovirus (Adv) belongs to the Adenovirus family. Adv can proliferate and cause disease in cells of the respiratory tract, gastrointestinal tract, urethra, and conjunctiva. It is mainly infected through the gastrointestinal tract, respiratory tract or close contact, especially in swimming pools with insufficient disinfection, which can increase the chance of transmission and cause outbreaks[1-2]. Adv mainly infects children. The gastrointestinal tract infections in children are mainly type 40 and 41 in group F. Most of them have no clinical symptoms, and some cause diarrhea in children. Its mechanism of action is to invade the small intestinal mucosa of children, making the intestinal mucosal epithelial cells smaller and shorter, and the cells degenerate and dissolve, resulting in intestinal absorption dysfunction and diarrhea. Abdominal pain and bloating may also occur, and in severe cases, the respiratory system, central nervous system, and extraintestinal organs such as liver, kidney, and pancreas may be involved and the disease may be aggravated.

Technical Parameters

Storage

≤-18℃

Shelf-life 12 months
Specimen Type stool 
Ct ≤38
CV <5.0%
LoD 300Copies/mL
Specificity Repeatability: Use the kits to detect the company repeatability reference. Repeat the test for 10 times and CV≤5.0%.

Specificity: Use the kits to test the standardized company negative reference, the results should meet the corresponding requirements

Applicable Instruments Applied Biosystems 7500 Real-Time PCR Systems,

Applied Biosystems 7500 Fast Real-Time PCR Systems,

QuantStudio®5 Real-Time PCR Systems,

SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.),

LightCycler®480 Real-Time PCR system,

LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology),

MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.)

Work Flow

Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. are recommended for the sample extraction and the subsequent steps should be conducted in strict accordance with the IFU of the Kit. 


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