West Nile Virus Nucleic Acid

Short Description:

This kit is used to detect West Nile virus nucleic acid in serum samples.


Product Detail

Product Tags

Product name

HWTS-FE041-West Nile Virus Nucleic Acid Detection Kit(Fluorescence PCR)

Epidemiology

West Nile virus is a member of the family Flaviviridae, genus Flavivirus, and is in the same genus as Japanese encephalitis virus, dengue virus, yellow fever virus, St. Louis encephalitis virus, hepatitis C virus, etc. In recent years, West Nile fever has caused epidemics in North America, Europe, the Middle East, and Southeast Asia, and has become the largest infectious disease currently affecting the United States. West Nile virus is transmitted through birds as reservoir hosts, and humans are infected through the bites of bird feeding (ornithophilic) mosquitoes such as Culex. Humans, horses, and other mammals become ill after being bitten by mosquitoes infected with West Nile virus. Mild cases may present with flu-like symptoms such as fever and headache, while severe cases may present with central nervous system symptoms or even death[1-3]. In recent years, due to the deepening of international exchanges and cooperation, exchanges between countries have become frequent, and the number of travelers has increased year by year. At the same time, due to factors such as the migration of migratory birds, the probability of West Nile fever being introduced into China has increased[4].

Technical Parameters

Storage

-18℃

Shelf-life 12 months
Specimen Type serum samples
CV ≤5.0%
LoD 500 Copies/μL
Applicable Instruments Applicable to type I detection reagent:

Applied Biosystems 7500 Real-Time PCR Systems, 

QuantStudio®5 Real-Time PCR Systems, 

SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.),

LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology),

MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.),

BioRad CFX96 Real-Time PCR System,

BioRad CFX Opus 96 Real-Time PCR System.

Applicable to type II detection reagent:

EudemonTM AIO800 (HWTS-EQ007) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Kit(YD315-R) manufactured by Tiangen Biotech(Beijing) Co., Ltd.


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