Respiratory Pathogens Combined

Short Description:

This kit is used for the in vitro qualitative detection of influenza A virus, influenza B virus, respiratory syncytial virus, adenovirus, human rhinovirus and mycoplasma pneumoniae nucleic acids in human nasopharyngeal swabs and oropharyngeal swab samples. The test results can be used for aid to the diagnosis of respiratory pathogen infections, and provide auxiliary molecular diagnostic basis for the diagnosis and treatment of respiratory pathogen infections.


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Respiratory Pathogens Combined Detail:

Product name

HWTS-RT050-Six Kinds of Respiratory Pathogen Nucleic Acid Detection Kit (Fluorescence PCR)

Epidemiology

Influenza, commonly known as 'flu', is an acute respiratory infectious disease caused by influenza virus, which is highly infectious and is mainly transmitted by coughing and sneezing.

Respiratory syncytial virus (RSV) is an RNA virus, belonging to the paramyxoviridae family.

Human adenovirus (HAdV) is a double stranded DNA virus without envelope. At least 90 genotypes have been found, which can be divided into 7 subgenera A-G.

Human rhinovirus (HRV) is a member of the Picornaviridae family and the Enterovirus genus.

Mycoplasma pneumoniae (MP) is a pathogenic microorganism that is between bacteria and viruses in size.

Channel

Channel PCR-Mix A PCR-Mix B
FAM Channel IFV A HAdV
VIC/HEX Channel HRV IFV B
CY5 Channel RSV MP
ROX Channel Internal Control Internal Control

Technical Parameters

Storage

-18℃

Shelf-life 12 months
Specimen Type Oropharyngeal swab
Ct ≤35
LoD 500Copies/mL
Specificity 1.The cross-reactivity test results showed that there was no cross reaction between the kit and human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, Parainfluenza virus types 1, 2, and 3, Chlamydia pneumoniae, human metapneumovirus, Enterovirus A, B, C, D, Epstein-Barr virus, Measles virus, human cytomegalovirus, Rotavirus, Norovirus, Mumps virus, Varicella-zoster virus, Legionella, Bordetella pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata, Pneumocystis jiroveci, Cryptococcus neoformans and human genomic nucleic acids.

2.Anti-interference ability: Mucin (60mg/mL), 10% (v/v) human blood, phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (with preservatives) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone ( 2mg/mL), Fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha-interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (40μg/mL), Meropenem (200mg/mL), levofloxacin (10μg/mL), and tobramycin (0.6mg/mL) were selected for the interference test, and the results showed that the interfering substances at the above concentrations had no interference reaction to the test results of pathogens.

Applicable Instruments Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler®480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection Systems

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System

Total PCR Solution

Six Kinds of Respiratory Pathogen Nucleic Acid Detection Kit 
(Fluorescence PCR)

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    5 Stars By Poppy from The Swiss - 2018.12.10 19:03
    Goods just received, we are very satisfied, a very good supplier, hope to make persistent efforts to do better.
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