Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)-RUO
Product name
Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)-RUO
Epidemiology
Dengue fever (DF), which is induced by denguevirus (DENV) infection, is one of the most epidemic arbovirus infectious diseases. Its transmission medium includes Aedes aegypti and Aedes albopictus. DF is mainly prevalent in tropical and subtropical areas. DENV belongs to flavivirus under flaviviridae, and can be classified into 4 serotypes according to surface antigen. Clinical manifestations of DENV infection mainly include headache, fever, weakness, enlargement of lymph node, leukopenia and etc., and bleeding, shock, hepatic injury or even death in severe cases. In recent years, climate change, urbanization, quick development of tourism and other factors have provided more rapid and convenient conditions for transmitting and spreading of DF, leading to constant expansion of epidemic area of DF. Therefore, establishment of easy, specific and rapid etiological diagnosis method of DENV has important significance for clinical diagnosis of DF.
Technical Parameters
| Storage | 2-8℃ |
| Shelf-life | 12 months |
| Specimen Type | serum |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 500 Copies/mL |
| Specificity | Tests are negative for dengue virus negative serum samples; the interference test results show that when the bilirubin concentration in serum is not more than 168.2μmol/mL, the concentration of hemoglobin produced by hemolysis is not more than 130g/L, the blood lipid concentration is not more than 65mmol/mL, and the total IgG concentration in serum is not more than 5mg/mL, there is no effect on the detection of dengue virus; use Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Herpes virus, Eastern equine encephalitis virus, Hantaan virus, Bunia virus, West Nile virus, Zika virus and human genomic serum samples for cross-reactivity test, and the results show that there is no cross reaction between this kit and pathogens above. |
| Applicable Instruments | Type I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection System(FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) , BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System.
Type II: EudemonTM AIO800 (HWTS-EQ007) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
|
Work Flow
Reagents need but not provided:
Type I: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022).
Type II: Macro & Micro-Test General DNA/RNA Kit(HWTS-3023-8), can be used with EudemonTM AIO800 (HWTS-EQ007) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Before using the Type II test reagent, the customer has a supporting extraction reagent and amplification instrument, and the engineer will guide the customer to carry out the experimental operation when using the first time.
Consumables need but not provided: DNase/RNase Free Tips, disposable gloves, DNase/RNase Free EP tubes, centrifuge, magnetic stand, 8-tube strips for PCR.
