Factory Supply Malaria Pf Ag Detection Kit - Malaria Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test

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Factory Supply Malaria Pf Ag Detection Kit - Malaria Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

Plasmodium Nucleic Acid Detection Kit(Fluorescence PCR)
Freeze-dried Plasmodium Nucleic Acid Detection Kit (Fluorescence PCR)

Certificate

Epidemiology

Malaria (Mal for short) is caused by Plasmodium, which is a single-celled eukaryotic organism, including Plasmodium falciparum Welch, Plasmodium vivax Grassi & Feletti, Plasmodium malariae Laveran, and Plasmodium ovale Stephens. It is a mosquito-borne and blood-borne parasitic disease that seriously endangers human health.

Of the parasites that cause malaria in humans, Plasmodium falciparum Welch is the deadliest. The incubation period of different malaria parasites is different, the shortest is 12-30 days, and the longer one can reach about 1 year. After the paroxysm of malaria, symptoms such as chills and fever may appear. The patients may have anemia and splenomegaly. The serious patients may have coma, severe anemia, acute renal failure which may lead to the death of patients^[1]. Malaria is distributed worldwide, mainly in tropical and subtropical regions such as Africa, Central America, and South America^[2].

Features

● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.
● High sensitivity: 5 Copies/μL.
● Two options: lyophilized product & liquid product.

Channel

FAM: Plasmodium nucleic acid
VIC (HEX): Internal control

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	2 mins	No
2	1 cycle	95℃	5 mins	No
3	40 cycles	95℃	10 s	No
3	40 cycles	58℃	30 s	Yes

Technical Parameters

Storage	≤-18℃ in dark
Shelf-life	24 months
Specimen Type	Whole blood, dried blood spots
Ct	≤38
CV	≤5.0％
LoD	5Copies/μL
Repeatability	Detect the company repeatability reference and calculate the coefficient of variation CV of Plasmodium detection Ct and the result≤ 5% (n=10).
Specificity	No cross reactivity with influenza A H1N1 virus, H3N2 influenza virus, influenza B virus, dengue fever virus, encephalitis B virus, respiratory syncytial virus, meningococcus, parainfluenza virus, rhinovirus, toxic bacillary dysentery, staphylococcus aureus, escherichia coli, streptococcus pneumoniae or klebsiella pneumoniae, salmonella typhi, and rickettsia tsutsugamushi, and the test results are all negative.
Applicable Instruments	It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems
ABI 7500 Real-Time PCR Systems
ABI 7500 Fast Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems
LightCycler®480 Real-Time PCR Systems
LineGene 9600 Plus Real-Time PCR Detection Systems
MA-6000 Real-Time Quantitative Thermal Cycler
BioRad CFX96 Real-Time PCR System
BioRad CFX Opus 96 Real-Time PCR System

Main Components

Plasmodium Nucleic Acid Detection Kit(Fluorescence PCR)

Component	Catalogue Number		Quantity	Component Description
	HWTS-OT074A	HWTS-OT074B
	Specification(20 tests/kit)	Specification(50 tests/kit)
Mal Reaction Buffer	400μL/vial	1 mL/vial	1 vial	Contains Plasmodium, internal control specific primers, fluorescent probes, Taq enzyme, UDG enzyme, reaction buffer, etc.
Mal Positive Control	400μL/vial	1 mL/vial	1 vial	Mixture of Plasmodium and internal control template
Mal Blank Control	400μL/vial	1 mL/vial	1 vial	DNase/RNase free H₂O

Freeze-dried Plasmodium Nucleic Acid Detection Kit (Fluorescence PCR)

Component	Character	Catalogue Number		Component Description
		HWTS-OT054A	HWTS-OT054B
		Specification/Quantity(20 tests/kit)	Specification/Quantity (50 tests/kit)
Mal Reaction Buffer	lyophilized	1 bottle	1 bottle	Contains Plasmodium, internal control specific primers, fluorescent probes, Taq enzyme, UDG enzyme, reaction buffer, etc.
Mal Positive Control	lyophilized	1 vial	1 vial	Mixture of Plasmodium and internal control template
Mal Blank Control	Liquid	1 vial, 600μL/vial	1 vial, 600μL/vial	DNase/RNase free H₂O
Reconstituted Solution	Liquid	1 vial, 1mL/vial	1 vial, 1.45mL/vial	DNase/RNase free H₂O, etc.

References

[1] Organization W H. World Malaria Report 2014 (PDF)[J]. 2015.
[2] Zwaenepoel B, Jan Philippé, Callens S. Screening naar malaria: LAMP-test als sensitiever alternatief voor de dikdruppeltest en implementatie hiervan in het UZ Gent[J]. Tijdschrift voor Geneeskunde, 2019, 75:914.

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