Chinese wholesale Malaria - Malaria Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test
Chinese wholesale Malaria - Malaria Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:
Product name
Plasmodium Nucleic Acid Detection Kit(Fluorescence PCR)
Freeze-dried Plasmodium Nucleic Acid Detection Kit (Fluorescence PCR)
Certificate
CE
Epidemiology
Malaria (Mal for short) is caused by Plasmodium, which is a single-celled eukaryotic organism, including Plasmodium falciparum Welch, Plasmodium vivax Grassi & Feletti, Plasmodium malariae Laveran, and Plasmodium ovale Stephens. It is a mosquito-borne and blood-borne parasitic disease that seriously endangers human health.
Of the parasites that cause malaria in humans, Plasmodium falciparum Welch is the deadliest. The incubation period of different malaria parasites is different, the shortest is 12-30 days, and the longer one can reach about 1 year. After the paroxysm of malaria, symptoms such as chills and fever may appear. The patients may have anemia and splenomegaly. The serious patients may have coma, severe anemia, acute renal failure which may lead to the death of patients[1]. Malaria is distributed worldwide, mainly in tropical and subtropical regions such as Africa, Central America, and South America[2].
Features
● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.
● High sensitivity: 5 Copies/μL.
● Two options: lyophilized product & liquid product.
Channel
FAM: Plasmodium nucleic acid
VIC (HEX): Internal control
PCR Amplification Conditions Setting
Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
1 | 1 cycle | 50℃ | 2 mins | No |
2 | 1 cycle | 95℃ | 5 mins | No |
3 | 40 cycles | 95℃ | 10 s | No |
58℃ | 30 s | Yes |
Technical Parameters
Storage | ≤-18℃ in dark |
Shelf-life | 24 months |
Specimen Type | Whole blood, dried blood spots |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 5Copies/μL |
Repeatability | Detect the company repeatability reference and calculate the coefficient of variation CV of Plasmodium detection Ct and the result≤ 5% (n=10). |
Specificity | No cross reactivity with influenza A H1N1 virus, H3N2 influenza virus, influenza B virus, dengue fever virus, encephalitis B virus, respiratory syncytial virus, meningococcus, parainfluenza virus, rhinovirus, toxic bacillary dysentery, staphylococcus aureus, escherichia coli, streptococcus pneumoniae or klebsiella pneumoniae, salmonella typhi, and rickettsia tsutsugamushi, and the test results are all negative. |
Applicable Instruments | It can match the mainstream fluorescent PCR instruments on the market. |
SLAN-96P Real-Time PCR Systems | |
ABI 7500 Real-Time PCR Systems | |
ABI 7500 Fast Real-Time PCR Systems | |
QuantStudio®5 Real-Time PCR Systems | |
LightCycler®480 Real-Time PCR Systems | |
LineGene 9600 Plus Real-Time PCR Detection Systems | |
MA-6000 Real-Time Quantitative Thermal Cycler | |
BioRad CFX96 Real-Time PCR System | |
BioRad CFX Opus 96 Real-Time PCR System |
Main Components
Plasmodium Nucleic Acid Detection Kit(Fluorescence PCR)
Component |
Catalogue Number |
Quantity | Component Description | |
HWTS-OT074A | HWTS-OT074B | |||
Specification(20 tests/kit) | Specification(50 tests/kit) | |||
Mal Reaction Buffer | 400μL/vial | 1 mL/vial | 1 vial | Contains Plasmodium, internal control specific primers, fluorescent probes, Taq enzyme, UDG enzyme, reaction buffer, etc. |
Mal Positive Control | 400μL/vial | 1 mL/vial | 1 vial | Mixture of Plasmodium and internal control template |
Mal Blank Control | 400μL/vial | 1 mL/vial | 1 vial | DNase/RNase free H2O |
Freeze-dried Plasmodium Nucleic Acid Detection Kit (Fluorescence PCR)
Component | Character |
Catalogue Number |
Component Description | |
HWTS-OT054A | HWTS-OT054B | |||
Specification/Quantity(20 tests/kit) | Specification/Quantity (50 tests/kit) | |||
Mal Reaction Buffer | lyophilized | 1 bottle | 1 bottle | Contains Plasmodium, internal control specific primers, fluorescent probes, Taq enzyme, UDG enzyme, reaction buffer, etc. |
Mal Positive Control | lyophilized | 1 vial | 1 vial | Mixture of Plasmodium and internal control template |
Mal Blank Control | Liquid | 1 vial, 600μL/vial | 1 vial, 600μL/vial | DNase/RNase free H2O |
Reconstituted Solution | Liquid | 1 vial, 1mL/vial | 1 vial, 1.45mL/vial | DNase/RNase free H2O, etc. |
References
[1] Organization W H. World Malaria Report 2014 (PDF)[J]. 2015.
[2] Zwaenepoel B, Jan Philippé, Callens S. Screening naar malaria: LAMP-test als sensitiever alternatief voor de dikdruppeltest en implementatie hiervan in het UZ Gent[J]. Tijdschrift voor Geneeskunde, 2019, 75:914.
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We attempt for excellence, company the customers, hopes to be the top cooperation team and dominator company for personnel, suppliers and customers, realizes price share and continual marketing for Chinese wholesale Malaria - Malaria Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test , The product will supply to all over the world, such as: Lebanon, Colombia, Malaysia, Based on our guiding principle of quality is the key to development, we continually strive to exceed our customers' expectations. As such, we sincerely invite all interested companies to contact us for future cooperation, We welcome old and new customers to hold hands together for exploring and developing; For more information, be sure to feel free to contact us. Thanks. Advanced equipment, strict quality control, customer-orientation service, initiative summary and improvement of defects and extensive industry experience enable us to guarantee more customer satisfaction and reputation which, in return, brings us more orders and benefits. If you are interested in any of our merchandise, make sure you feel free to contact us. Inquiry or visit to our company are warmly welcome. We sincerely hope to start a win-win and friendly partnership with you. You can see more details in our website.
By Eartha from Luxembourg - 2018.06.03 10:17
The supplier cooperation attitude is very good, encountered various problems, always willing to cooperate with us, to us as the real God.
By Eve from Angola - 2017.11.01 17:04