Wholesale Fsh Rapid Test Kit - Macro & Micro-Test Sample Release Reagent – Macro & Micro-Test
Wholesale Fsh Rapid Test Kit - Macro & Micro-Test Sample Release Reagent – Macro & Micro-Test Detail:
		Product name
	
Macro & Micro-Test Sample Release Reagent
		Certificate
	
CE, FDA, NMPA
		Features
	
Rapid: only 5 mins.
Easy: only 3 steps (1.add the sample 2.Mix upside and down 10 times 3. Leave it for 5 mins).
		Main components
	
| Name | Main components | Component specifications | Quantity | 
| Sample Release reagent | Dithiothreitol, sodium dodecyl sulfate (SDS), RNase inhibitor, surfactant, purified water | 0.5mL/Vial | 50 Vial | 
Note: Components in different batches of kits are not interchangeable.
		Storage conditions and shelf life
	
Store and transport at room temperature. The shelf life is 24 months.
		Applicable instruments
	
Instruments and equipment during sample processing, such as pipettes, vortex mixers, water baths, etc.
		Sample requirements
	
Freshly collected oropharyngeal swabs, nasopharyngeal swabs.
		Precision
	
When this kit is used for extraction from the in-house precision reference CV for 10 replicates, the coefficient of variation (CV, %) of Ct value is not more than 10%.
		Inter-batch difference
	
When the in-house precision reference is tested on three batches of kits under trial production upon repeated extraction and, the coefficient of variation (CV, %) of Ct value is not more than 10%.
		Performance comparison
	
● Extraction efficiency comparsion
| 
 Efficiency comparsion of magnetic beads method and sample releaser  | 
||||
| 
 concentration  | 
 magnetic beads method  | 
 sample releaser  | 
||
| 
 orfab  | 
 N  | 
 orfab  | 
 N  | 
|
| 
 20000  | 
 28.01  | 
 28.76  | 
 28.6  | 
 29.15  | 
| 
 2000  | 
 31.53  | 
 31.9  | 
 32.35  | 
 32.37  | 
| 
 500  | 
 33.8  | 
 34  | 
 35.25  | 
 35.9  | 
| 
 200  | 
 35.25  | 
 35.9  | 
 35.83  | 
 35.96  | 
| 
 100  | 
 36.99  | 
 37.7  | 
 38.13  | 
 undet  | 
The extraction efficiency of sample releaser was similar to that of magnetic beads method, and the concentration of pathogen could be 200Copies/mL.
● CV value comparison
| 
 Repeatability of sample releaser extraction  | 
||
| concentration:5000Copies/mL | 
 ORF1ab  | 
 N  | 
| 
 30.17  | 
 30.38  | 
|
| 
 30.09  | 
 30.36  | 
|
| 
 30.36  | 
 30.26  | 
|
| 
 30.03  | 
 30.48  | 
|
| 
 30.14  | 
 30.45  | 
|
| 
 30.31  | 
 30.16  | 
|
| 
 30.38  | 
 30.7  | 
|
| 
 30.72  | 
 30.79  | 
|
| CV | 
 0.73%  | 
 0.69%  | 
When tested at 5,000 copies /mL, the CV of orFab and N was 0.73% and 0.69%, respectively.
				
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