Wholesale Dealers of Dengue Ns1 Rapid Test - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test

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Wholesale Dealers of Dengue Ns1 Rapid Test - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)
Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

Certificate

CE

Epidemiology

Dengue fever (DF), which is induced by denguevirus (DENV) infection, is one of the most epidemic arbovirus infectious diseases. DENV belongs to flavivirus under flaviviridae, and can be classified into 4 serotypes according to surface antigen. Its transmission medium includes Aedes aegypti and Aedes albopictus, prevalent mainly in tropical and subtropical areas.

Clinical manifestations of DENV infection mainly include headache, fever, weakness, enlargement of lymph node, leukopenia and etc., and bleeding, shock, hepatic injury or even death in severe cases. In recent years, climate change, urbanization, quick development of tourism and other factors have provided more rapid and convenient conditions for transmitting and spreading of DF, leading to constant expansion of epidemic area of DF.

Features

● Multiplex PCR Amplification Technology.
● Sub-Package: Safer and easier.
● High sensitivity: 500 Copies/mL.
● High specificity: No cross reactivity with common pathogens causing febrile and hemorrhagic diseases.

Channel

FAM: Dengue Virus I
VIC(HEX): Dengue Virus II
ROX: Dengue Virus III
CY5: Dengue Virus IV

PCR Amplification Conditions Setting

Step Cycles Temperature Time Collect Fluorescent Signals or Not
1 1 cycle 50℃ 30mins No
2 1 cycle 95℃ 15mins No
3 40 cycles 95℃ 15s No
60℃ 45s Yes

Technical Parameters

Storage
Liquid ≤-18℃ in dark; lyophilization:≤30℃ in dark
Shelf-life 12 months
Specimen Type Fresh Serum
Ct ≤38
CV ≤5.0%
LoD 500 Copies/mL

Specificity: Perform cross reaction tests of Japanese encephalitis virus, Forest encephalitis virus, severe fever with thrombocytopenia syndrome, Xinjiang hemorrhagic fever, hantaan virus, hepatitis C virus, influenza A virus, influenza B virus and etc. No cross reaction is detected.

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
ABI 7500 Fast Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.
BioRad CFX96 Real-Time PCR System.
BioRad CFX Opus 96 Real-Time PCR System.

Main Components

Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

Component Catalogue Number Quantity Component Description
HWTS-FE034A HWTS-FE034B
Specification(20 tests/kit) Specification(50 tests/kit)
DENV Reaction Buffer 394μL/vial 985μL/vial 1 vial Dengue virus I/II/III/IV primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc.
DENV Enzyme Mix 6μL/vial 15μL/vial 1 vial Reverse transcriptase, etc.
DENV Positive Control 400μL/vial 1 mL/vial 1 vial Mixture of DENV I/II/III/IV templates
DENV Blank Control 400μL/vial 1 mL/vial 1 vial DNase/RNase free H2O

Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

Component Character Catalogue Number Component Description
HWTS-FE004B HWTS-FE004C
Specification/Quantity(20 tests/kit) Specification/Quantity (50 tests/kit)
DENV Reaction Buffer lyophilized 1 bottle 1 bottle dengue virus I/II/III/IV primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc.
DENV Positive Control lyophilized 1 vial 1 vial Mixture of DENV I/II/III/IV templates
DENV Blank Control Liquid 1 vial, 600μL/vial 1 vial, 600μL/vial DNase/RNase free H2O
Reconstituted Solution Liquid 1 vial, 1mL/vial 1 vial, 1.45mL/vial DNase/RNase free H2O, etc.

Total PCR Solution

Dengue Virus I II III IV Nucleic Acid Detection Kit6

Reference

[1] Shu P Y, Chang S F, Kuo Y C, et al. Development of Group- and Serotype-Specific One-Step Sybr Green I-Based Real-Time Reverse Transcription-PCR Assay for Dengue Virus[J]. Journal of Clinical Microbiology, 2003, 41(6):2408-2416.
[2] Paudel D, Jarman R , Limkittikul K, et al. Comparison of real-time SYBR green dengue assay with real-time taqman RT-PCR dengue assay and the conventional nested PCR for diagnosis of primary and secondary dengue infection[J]. North American Journal of Medical Sciences, 2011, 3(10):478-485.
[3] Waggoner J J, Ballesteros G, Gresh L, et al. Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection[J]. Journal of Clinical Virology the Official Publication of the Pan American Society for Clinical Virology, 2016:57-61.
[4] Yao J X, Zhao W, Zhang L, et al. Research of Real-Time Fluorescence PCR Detection on Dengue Virus[J]. West China Medical Journal, 2009.


Product detail pictures:

Wholesale Dealers of Dengue Ns1 Rapid Test - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Wholesale Dealers of Dengue Ns1 Rapid Test - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Wholesale Dealers of Dengue Ns1 Rapid Test - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Wholesale Dealers of Dengue Ns1 Rapid Test - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

Wholesale Dealers of Dengue Ns1 Rapid Test - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures


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Our mission should be to turn out to be an innovative supplier of high-tech digital and communication devices by furnishing benefit added design and style, world-class manufacturing, and repair capabilities for Wholesale Dealers of Dengue Ns1 Rapid Test - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test , The product will supply to all over the world, such as: Nepal, Thailand, panama, By adhering to the principle of human oriented, winning by quality, our company sincerely welcomes merchants from at home and abroad to visit us, talk business with us and jointly create a brilliant future.
  • The customer service staff's attitude is very sincere and the reply is timely and very detailed, this is very helpful for our deal,thank you.
    5 Stars By Amy from Dubai - 2018.06.12 16:22
    The company's products can meet our diverse needs, and the price is cheap, the most important is that the quality is also very nice.
    5 Stars By Eartha from Sydney - 2018.09.08 17:09
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