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    • Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test

    Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test Featured Image
    • Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test
    • Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test
    • Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test
    • Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test
    • Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test

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    Well-run gear, qualified revenue workforce, and superior after-sales companies; We've been also a unified huge loved ones, anyone persist with the organization benefit unification, determination, tolerance for Ffn Test, Hcv Genotype Assay, Hbv Quantitative, At present, company name has more than 4000 kinds of products and gained good reputation and big shares on market domestic and overseas.
    Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

    Product name

    Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)
    Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

    Certificate

    CE

    Epidemiology

    Dengue fever (DF), which is induced by denguevirus (DENV) infection, is one of the most epidemic arbovirus infectious diseases. DENV belongs to flavivirus under flaviviridae, and can be classified into 4 serotypes according to surface antigen. Its transmission medium includes Aedes aegypti and Aedes albopictus, prevalent mainly in tropical and subtropical areas.

    Clinical manifestations of DENV infection mainly include headache, fever, weakness, enlargement of lymph node, leukopenia and etc., and bleeding, shock, hepatic injury or even death in severe cases. In recent years, climate change, urbanization, quick development of tourism and other factors have provided more rapid and convenient conditions for transmitting and spreading of DF, leading to constant expansion of epidemic area of DF.

    Features

    ● Multiplex PCR Amplification Technology.
    ● Sub-Package: Safer and easier.
    ● High sensitivity: 500 Copies/mL.
    ● High specificity: No cross reactivity with common pathogens causing febrile and hemorrhagic diseases.

    Channel

    FAM: Dengue Virus I
    VIC(HEX): Dengue Virus II
    ROX: Dengue Virus III
    CY5: Dengue Virus IV

    PCR Amplification Conditions Setting

    Step Cycles Temperature Time Collect Fluorescent Signals or Not
    1 1 cycle 50℃ 30mins No
    2 1 cycle 95℃ 15mins No
    3 40 cycles 95℃ 15s No
    60℃ 45s Yes

    Technical Parameters

    Storage
    Liquid ≤-18℃ in dark; lyophilization:≤30℃ in dark
    Shelf-life 12 months
    Specimen Type Fresh Serum
    Ct ≤38
    CV ≤5.0％
    LoD 500 Copies/mL

    Specificity: Perform cross reaction tests of Japanese encephalitis virus, Forest encephalitis virus, severe fever with thrombocytopenia syndrome, Xinjiang hemorrhagic fever, hantaan virus, hepatitis C virus, influenza A virus, influenza B virus and etc. No cross reaction is detected.

    Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
    SLAN-96P Real-Time PCR Systems.
    ABI 7500 Real-Time PCR Systems.
    ABI 7500 Fast Real-Time PCR Systems.
    QuantStudio®5 Real-Time PCR Systems.
    LightCycler®480 Real-Time PCR Systems.
    LineGene 9600 Plus Real-Time PCR Detection Systems.
    MA-6000 Real-Time Quantitative Thermal Cycler.
    BioRad CFX96 Real-Time PCR System.
    BioRad CFX Opus 96 Real-Time PCR System.

    Main Components

    Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

    Component Catalogue Number Quantity Component Description
    HWTS-FE034A HWTS-FE034B
    Specification(20 tests/kit) Specification(50 tests/kit)
    DENV Reaction Buffer 394μL/vial 985μL/vial 1 vial Dengue virus I/II/III/IV primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc.
    DENV Enzyme Mix 6μL/vial 15μL/vial 1 vial Reverse transcriptase, etc.
    DENV Positive Control 400μL/vial 1 mL/vial 1 vial Mixture of DENV I/II/III/IV templates
    DENV Blank Control 400μL/vial 1 mL/vial 1 vial DNase/RNase free H2O

    Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

    Component Character Catalogue Number Component Description
    HWTS-FE004B HWTS-FE004C
    Specification/Quantity(20 tests/kit) Specification/Quantity (50 tests/kit)
    DENV Reaction Buffer lyophilized 1 bottle 1 bottle dengue virus I/II/III/IV primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc.
    DENV Positive Control lyophilized 1 vial 1 vial Mixture of DENV I/II/III/IV templates
    DENV Blank Control Liquid 1 vial, 600μL/vial 1 vial, 600μL/vial DNase/RNase free H2O
    Reconstituted Solution Liquid 1 vial, 1mL/vial 1 vial, 1.45mL/vial DNase/RNase free H2O, etc.

    Total PCR Solution

    Dengue Virus I II III IV Nucleic Acid Detection Kit6

    Reference

    [1] Shu P Y, Chang S F, Kuo Y C, et al. Development of Group- and Serotype-Specific One-Step Sybr Green I-Based Real-Time Reverse Transcription-PCR Assay for Dengue Virus[J]. Journal of Clinical Microbiology, 2003, 41(6):2408-2416.
    [2] Paudel D, Jarman R , Limkittikul K, et al. Comparison of real-time SYBR green dengue assay with real-time taqman RT-PCR dengue assay and the conventional nested PCR for diagnosis of primary and secondary dengue infection[J]. North American Journal of Medical Sciences, 2011, 3(10):478-485.
    [3] Waggoner J J, Ballesteros G, Gresh L, et al. Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection[J]. Journal of Clinical Virology the Official Publication of the Pan American Society for Clinical Virology, 2016:57-61.
    [4] Yao J X, Zhao W, Zhang L, et al. Research of Real-Time Fluorescence PCR Detection on Dengue Virus[J]. West China Medical Journal, 2009.

    Product detail pictures:

    Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

    Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

    Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

    Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

    Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test detail pictures

    Related Product Guide:
    Innovation, excellent and reliability are the core values of our firm. These principles today more than ever form the basis of our success as an internationally active mid-size corporation for Well-designed Dengue Ns1 Antigen - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test , The product will supply to all over the world, such as: Bangladesh, Angola, Turin, We have more than 200 staff including experienced managers, creative designers, sophisticated engineers and skilled workers. Through hard work of all employees for the past 20 years own company grew stronger and stronger. We always apply the client first principle. We also always fulfill all contracts to the point and therefore enjoy excellent reputation and trust among our customers. You are very welcome to personally visit our company.We hope to start a business partnership on the basis of mutual benefit and successful development . For more information please do no hesitate to contact us..
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    5 Stars By Ida from Zambia - 2018.11.02 11:11
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