Nucleic Acid Detection Kit based on Enzymatic Probe Isothermal Amplification (EPIA) for Group B Streptococcus – Macro & Micro-Test
Nucleic Acid Detection Kit based on Enzymatic Probe Isothermal Amplification (EPIA) for Group B Streptococcus – Macro & Micro-Test Detail:
Product Name
HWTS-UR019A-Freeze-dried Chlamydia Trachomatis, Ureaplasma Urealyticum and Neisseria Gonorrhoeae Nucleic Acid Detection Kit (Fluorescence PCR)
HWTS-UR019D-Chlamydia Trachomatis, Ureaplasma Urealyticum and Neisseria Gonorrhoeae Nucleic Acid Detection Kit (Fluorescence PCR)
Intended Use
This kit is suitable for the qualitative detection of common pathogens in urogenital infections in vitro, including Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU), and Neisseria gonorrhoeae (NG).
Epidemiology
Sexually transmitted diseases (STD) remain one of the major threats to global public health security, which can lead to infertility, premature fetal birth, tumorigenesis and various serious complications. There are many kinds of STD pathogens, including types like bacteria, viruses, chlamydia, mycoplasma and spirochetes, etc., and the common species are Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Herpes simplex virus type 1, herpes simplex virus type 2, Mycoplasma hominis, Mycoplasma genitalium, etc.
Channel
FAM: Chlamydia trachomatis (CT)
VIC(HEX): Ureaplasma urealyticum (UU)
ROX: Neisseria gonorrhoeae (NG)
CY5: Internal Control
PCR Amplification Conditions Setting
Liquid product:
|
Step |
Cycles |
Temperature |
Time |
Collect Fluorescent Signals or Not |
|
1 |
1 cycle |
50℃ |
5mins |
No |
|
2 |
1 cycle |
95℃ |
10mins |
No |
|
3 |
40 cycles |
95℃ |
15 secs |
No |
|
58℃ |
31 secs |
Yes |
Lyophilized product:
| Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
| 1 | 1 cycle | 50℃ | 5mins | No |
| 2 | 1 cycle | 95℃ | 10mins | No |
| 3 | 40 cycles | 95℃ | 15 secs | No |
| 58℃ | 30 secs | Yes |
Technical Parameters
Storage:
Liquid: ≤-18℃ In dark; Lyophilized: ≤30℃ In dark
Shelf-life: 12 months
Specimen Type: Urethral secretions, cervical secretions
Ct: ≤38
CV: ≤5.0%
LoD:
Liquid: 50 Copies/reaction; Lyophilized: 500Copies/mL
Specificity: There is no cross-reactivity for detecting other STD-infected pathogens, such as Treponema pallidum, etc.
Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR System
QuantStudio® 5 Real-Time PCR Systems
SLAN-96P Real-Time PCR Systems
LightCycler®480 Real-Time PCR system
LineGene 9600 Plus Real-Time PCR Detection System
MA-6000 Real-Time Quantitative Thermal Cycler
BioRad CFX96 Real-Time PCR System
BioRad CFX Opus 96 Real-Time PCR System
Work Flow
Option 1.
Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).
Option 2.
Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd.
Product detail pictures:
Related Product Guide:
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