Manufactur standard Ebv Infection - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test

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Manufactur standard Ebv Infection - EB Virus Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

EB Virus Nucleic Acid Detection Kit(Fluorescence PCR)

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Epidemiology

EBV (Epstein-barr virus), or human herpesvirus type 4, is a common human herpesvirus^[1]. In recent years, a large number of studies have proved that EBV is associated with occurrence and development of nasopharyngeal cancer, Hodgkin’s disease, T/Natural killer celllymphoma, Burkitt’s lymphoma, breast cancer, gastric cancer and other malignant tumors. And it is also closely associated with post-transplantlymphoproliferative disorders, post-transplant smooth muscle tumor and acquired immunedeficiency syndrome(AIDS) related lymphoma, multiple sclerosis, primary central nervous system lymphoma or leiomyosarcoma^[2-4].

Features

● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 500Copies/mL.
● Convenient: One test for multiple results.

Channel

FAM: EBV
VIC (HEX): Internal control

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	2mins	No
2	1 cycle	95℃	5mins	No
3	40 cycles	95℃	15s	No
3	40 cycles	58℃	30s	Yes

Technical Parameters

Storage	≤-18℃ In dark
Shelf-life	12 months
Specimen Type	Whole blood, Plasma, Serum
Ct	≤38
CV	≤5.0％
LoD	500Copies/mL

Specificity: It has no cross-reactivity with other pathogens (such as human herpesvirus 1, 2, 3, 6, 7, 8, hepatitis B virus, cytomegalovirus, influenza A, etc.) or bacteria (Staphylococcus aureus, Candida albicans, etc.)

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.

Main Components

Catalogue Number	Component (50 tests/kit)	Specification	Quantity	Component Description
HWTS-OT061A	EB Reaction Buffer	1.175mL/vial	1 vial	Amplification reaction reagents, EB virus primer probes, internal reference primer probes, etc.
	EB Enzyme Mix	75μL/vial	1 vial	Taq enzyme, UDG enzyme, etc.
	EB Positive Control	600μL/vial	1 vial	Mixed solution of viral target gene and internal reference
	EB Blank Control	600μL/vial	1 vial	DNase/RNase free H₂O

Total PCR Solution

EB Virus Nucleic Acid Detection Kit(Fluorescence PCR)6

Reference

[1] ohen JI, Jaffe ES, Dale JK, et al. Characterization and treatment of chronic active Epstein-Barr virus disease: a 28-year experience in the United States[J]. Blood, 2011, 117(22): 5835-5849.
[2] Jahangiryan A, Kheirandish M, Samiee S, et al. Determination of Epstein-Barr virus (EBV) incidence in umbilical cord blood (UCB) and assessment of virus DNA via Real-time PCR[J]. Annals of Cancer Research and Therapy, 2021, 29(1):114-120.
[3] Petersson F. Nasopharyngeal carcinoma:a review[J].Semin Diagn Pathol, 2015, 32(1):54-73. [4]Chen J N, He D, Tang F, et al. Epstein-Barr virus-associated gastric carcinoma:a newly defined entity[J]. J Clin Gastroenterol, 2012, 46(4):262-271.

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Eb Virus