Manufactur standard Dengue Ns1 Ag Detection Kit - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test

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Manufactur standard Dengue Ns1 Ag Detection Kit - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:

Product name

Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)
Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

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Epidemiology

Dengue fever (DF), which is induced by denguevirus (DENV) infection, is one of the most epidemic arbovirus infectious diseases. DENV belongs to flavivirus under flaviviridae, and can be classified into 4 serotypes according to surface antigen. Its transmission medium includes Aedes aegypti and Aedes albopictus, prevalent mainly in tropical and subtropical areas.

Clinical manifestations of DENV infection mainly include headache, fever, weakness, enlargement of lymph node, leukopenia and etc., and bleeding, shock, hepatic injury or even death in severe cases. In recent years, climate change, urbanization, quick development of tourism and other factors have provided more rapid and convenient conditions for transmitting and spreading of DF, leading to constant expansion of epidemic area of DF.

Features

● Multiplex PCR Amplification Technology.
● Sub-Package: Safer and easier.
● High sensitivity: 500 Copies/mL.
● High specificity: No cross reactivity with common pathogens causing febrile and hemorrhagic diseases.

Channel

FAM: Dengue Virus I
VIC(HEX): Dengue Virus II
ROX: Dengue Virus III
CY5: Dengue Virus IV

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	30mins	No
2	1 cycle	95℃	15mins	No
3	40 cycles	95℃	15s	No
3	40 cycles	60℃	45s	Yes

Technical Parameters

Storage
Liquid	≤-18℃ in dark; lyophilization:≤30℃ in dark
Shelf-life	12 months
Specimen Type	Fresh Serum
Ct	≤38
CV	≤5.0％
LoD	500 Copies/mL

Specificity: Perform cross reaction tests of Japanese encephalitis virus, Forest encephalitis virus, severe fever with thrombocytopenia syndrome, Xinjiang hemorrhagic fever, hantaan virus, hepatitis C virus, influenza A virus, influenza B virus and etc. No cross reaction is detected.

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
ABI 7500 Fast Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.
BioRad CFX96 Real-Time PCR System.
BioRad CFX Opus 96 Real-Time PCR System.

Main Components

Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

Component	Catalogue Number		Quantity	Component Description
	HWTS-FE034A	HWTS-FE034B
	Specification(20 tests/kit)	Specification(50 tests/kit)
DENV Reaction Buffer	394μL/vial	985μL/vial	1 vial	Dengue virus I/II/III/IV primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc.
DENV Enzyme Mix	6μL/vial	15μL/vial	1 vial	Reverse transcriptase, etc.
DENV Positive Control	400μL/vial	1 mL/vial	1 vial	Mixture of DENV I/II/III/IV templates
DENV Blank Control	400μL/vial	1 mL/vial	1 vial	DNase/RNase free H₂O

Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

Component	Character	Catalogue Number		Component Description
		HWTS-FE004B	HWTS-FE004C
		Specification/Quantity(20 tests/kit)	Specification/Quantity (50 tests/kit)
DENV Reaction Buffer	lyophilized	1 bottle	1 bottle	dengue virus I/II/III/IV primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc.
DENV Positive Control	lyophilized	1 vial	1 vial	Mixture of DENV I/II/III/IV templates
DENV Blank Control	Liquid	1 vial, 600μL/vial	1 vial, 600μL/vial	DNase/RNase free H₂O
Reconstituted Solution	Liquid	1 vial, 1mL/vial	1 vial, 1.45mL/vial	DNase/RNase free H₂O, etc.

Total PCR Solution

Dengue Virus I II III IV Nucleic Acid Detection Kit6

Reference

[1] Shu P Y, Chang S F, Kuo Y C, et al. Development of Group- and Serotype-Specific One-Step Sybr Green I-Based Real-Time Reverse Transcription-PCR Assay for Dengue Virus[J]. Journal of Clinical Microbiology, 2003, 41(6):2408-2416.
[2] Paudel D, Jarman R , Limkittikul K, et al. Comparison of real-time SYBR green dengue assay with real-time taqman RT-PCR dengue assay and the conventional nested PCR for diagnosis of primary and secondary dengue infection[J]. North American Journal of Medical Sciences, 2011, 3(10):478-485.
[3] Waggoner J J, Ballesteros G, Gresh L, et al. Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection[J]. Journal of Clinical Virology the Official Publication of the Pan American Society for Clinical Virology, 2016:57-61.
[4] Yao J X, Zhao W, Zhang L, et al. Research of Real-Time Fluorescence PCR Detection on Dengue Virus[J]. West China Medical Journal, 2009.

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