Influenza B Virus Nucleic Acid Quantitative
Product name
HWTS-RT140-Influenza B Virus Nucleic Acid Quantitative Detection Kit(Fluorescence PCR)
Epidemiology
Influenza, commonly referred to as 'flu', is an acute respiratory infectious disease caused by influenza virus. It is highly contagious and spreads mainly through coughing and sneezing. It usually breaks out in spring and winter. There are three types: Influenza A (IFV A), Influenza B (IFV B), and Influenza C (IFV C), all of which belong to the Orthomyxoviridae family. The main causes of human diseases are Influenza A and B viruses, and they are single-strand negative-sense, segmented RNA viruses. Influenza B viruses are divided into two major lineages, Yamagata and Victoria. Influenza B viruses only have antigenic drift, and they evade the surveillance and clearance of the human immune system through their mutations. However, the evolution rate of influenza B virus is slower than that of influenza A virus, and influenza B virus can also cause human respiratory tract infection and lead to epidemics.
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | Oropharyngeal swab sample |
| CV | <5.0% |
| LoD | 500Copies/mL |
| Specificity |
Cross-reactivity: there is no cross reactivity between this kit and influenza A virus, adenovirus type 3, 7, human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, and HCoV-NL63, cytomegalovirus, enterovirus, parainfluenza virus, measles virus, human metapneumovirus, mumps virus, respiratory syncytial virus type B, rhinovirus, bordetella pertussis,chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus influenzae, jactobacillus, moraxella catarrhalis, avirulent mycobacterium tuberculosis, mycoplasma pneumoniae, neisseria meningitidis, neisseria gonorrhoeae, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, streptococcus salivarius and human genomic DNA. |
| Applicable Instruments | Applied Biosystems 7500 Real-Time PCR System,
Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. are recommended for the sample extraction and the subsequent steps should be conducted in strict accordance with the IFU of the Kit.
