High reputation Hpv 16 - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test
High reputation Hpv 16 - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test Detail:
Product name
28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR )
Certificate
CE
Epidemiology
Cervical cancer is one of the most common malignant tumors in the female reproductive tract. The studies have shown that persistent infection and multiple infections of human papillomavirus is one of the important cause of cervical cancer. At present, there is still a lack of recognized effective treatment methods for HPV. Therefore, early detection and early prevention of cervical HPV is the key to blocking canceration. The establishment of a simple, specific and rapid pathogenic diagnostic method is of great significance in the clinical diagnosis of cervical cancer.
Features
● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 25 Copies/reaction
● High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.
● Convenient: One test for multiple results.
Channel
| S/N | Channel | Type |
| PCR-Mix1 | FAM | 16, 18, 31, 56 |
| VIC(HEX) | Internal Control | |
| CY5 | 45, 51, 52, 53 | |
| ROX | 33, 35, 58, 66 | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| CY5 | 40, 42, 43, 82 | |
| ROX | 39, 59, 68, 73 |
PCR Amplification Conditions Setting
| Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
| 1 | 1 cycle | 50℃ | 2 mins | No |
| 2 | 1 cycle | 95℃ | 10 mins | No |
| 3 | 10 cycle | 95℃ | 15 s | No |
| 62℃ | 20 s | No | ||
| 4 | 30 cycle | 95℃ | 15 s | No |
| 58℃ | 30 s | Yes |
Technical Parameters
| Storage | ≤-18℃ in dark |
| Shelf-life | 12 months |
| Specimen Type | Cervical exfoliated cells |
| Ct | ≤28 |
| CV | ≤5.0％ |
| LoD | 25 Copies/reaction |
| Specificity | No cross-reactivity with common reproductive tract pathogens (such as ureaplasma urealyticum, genital tract chlamydia trachomatis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella and other HPV types not covered in the kit, etc). |
| Applicable Instruments | It can match the mainstream fluorescent PCR instruments on the market. |
| SLAN-96P Real-Time PCR Systems | |
| ABI 7500 Real-Time PCR Systems | |
| QuantStudio®5 Real-Time PCR Systems | |
| LightCycler®480 Real-Time PCR Systems | |
| LineGene 9600 Plus Real-Time PCR Detection Systems | |
| MA-6000 Real-Time Quantitative Thermal Cycler | |
Main Components
| Catalogue Number | Component (50 tests/kit) | Specification | Quantity | Component Description |
| HWTS-CC003A | HPV WELL1 | 1.3mL/vial | 1 vial | Mixture of specific primers and probes of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66 |
| HPV WELL2 | 1.3mL/vial | 1 vial | Mixture of specific primers and probes of HPV6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83 | |
| HPV Enzyme Mix | 55uL/vial | 1 vial | Mixture of Taq DNA polymerase, Uracil-DNA Glycosylase and glycerin | |
| HPV Positive Control WELL1 | 150μL/vial | 1 vial | Mixture of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66 templates and Internal Control template | |
| HPV Positive Control WELL2 | 150μL/vial | 1 vial | Mixture of 6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83 templates | |
| HPV Blank Control | 300μL/vial | 1 vial | RNase/DNase free H2O |
Total PCR Solution
Option 1.
Option 2.
Product detail pictures:
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Assume full duty to satisfy all demands of our clients; reach steady advancements by marketing the development of our purchasers; grow to be the final permanent cooperative partner of clientele and maximize the interests of customers for High reputation Hpv 16 - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test , The product will supply to all over the world, such as: Borussia Dortmund, Ghana, moldova, Our company always concentrate on the development of the international market. We've got a lot of customers in Russia , European countries, the USA, the Middle East countries and Africa countries. We always follow that quality is foundation while service is guarantee to meet all customers.
By Marguerite from Costa Rica - 2017.01.11 17:15
Good quality and fast delivery, it's very nice. Some products have a little bit problem, but the supplier replaced timely, overall, we are satisfied.
By Debby from Albania - 2017.04.28 15:45
