High reputation Hpv 16 - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test
High reputation Hpv 16 - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test Detail:
Product name
28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR )
Certificate
CE
Epidemiology
Cervical cancer is one of the most common malignant tumors in the female reproductive tract. The studies have shown that persistent infection and multiple infections of human papillomavirus is one of the important cause of cervical cancer. At present, there is still a lack of recognized effective treatment methods for HPV. Therefore, early detection and early prevention of cervical HPV is the key to blocking canceration. The establishment of a simple, specific and rapid pathogenic diagnostic method is of great significance in the clinical diagnosis of cervical cancer.
Features
● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 25 Copies/reaction
● High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.
● Convenient: One test for multiple results.
Channel
| S/N | Channel | Type |
| PCR-Mix1 | FAM | 16, 18, 31, 56 |
| VIC(HEX) | Internal Control | |
| CY5 | 45, 51, 52, 53 | |
| ROX | 33, 35, 58, 66 | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| CY5 | 40, 42, 43, 82 | |
| ROX | 39, 59, 68, 73 |
PCR Amplification Conditions Setting
| Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
| 1 | 1 cycle | 50℃ | 2 mins | No |
| 2 | 1 cycle | 95℃ | 10 mins | No |
| 3 | 10 cycle | 95℃ | 15 s | No |
| 62℃ | 20 s | No | ||
| 4 | 30 cycle | 95℃ | 15 s | No |
| 58℃ | 30 s | Yes |
Technical Parameters
| Storage | ≤-18℃ in dark |
| Shelf-life | 12 months |
| Specimen Type | Cervical exfoliated cells |
| Ct | ≤28 |
| CV | ≤5.0％ |
| LoD | 25 Copies/reaction |
| Specificity | No cross-reactivity with common reproductive tract pathogens (such as ureaplasma urealyticum, genital tract chlamydia trachomatis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella and other HPV types not covered in the kit, etc). |
| Applicable Instruments | It can match the mainstream fluorescent PCR instruments on the market. |
| SLAN-96P Real-Time PCR Systems | |
| ABI 7500 Real-Time PCR Systems | |
| QuantStudio®5 Real-Time PCR Systems | |
| LightCycler®480 Real-Time PCR Systems | |
| LineGene 9600 Plus Real-Time PCR Detection Systems | |
| MA-6000 Real-Time Quantitative Thermal Cycler | |
Main Components
| Catalogue Number | Component (50 tests/kit) | Specification | Quantity | Component Description |
| HWTS-CC003A | HPV WELL1 | 1.3mL/vial | 1 vial | Mixture of specific primers and probes of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66 |
| HPV WELL2 | 1.3mL/vial | 1 vial | Mixture of specific primers and probes of HPV6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83 | |
| HPV Enzyme Mix | 55uL/vial | 1 vial | Mixture of Taq DNA polymerase, Uracil-DNA Glycosylase and glycerin | |
| HPV Positive Control WELL1 | 150μL/vial | 1 vial | Mixture of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66 templates and Internal Control template | |
| HPV Positive Control WELL2 | 150μL/vial | 1 vial | Mixture of 6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83 templates | |
| HPV Blank Control | 300μL/vial | 1 vial | RNase/DNase free H2O |
Total PCR Solution
Option 1.
Option 2.
Product detail pictures:
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Our commission should be to provide our end users and clients with very best excellent and aggressive portable digital products and solutions for High reputation Hpv 16 - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test , The product will supply to all over the world, such as: Johannesburg, Sao Paulo, Uruguay, Our objective is to supply first step products and best service for our customers, thus we are sure you must have a margin benefit through cooperating with us. If you are interested in any of our products or would like to discuss a custom order, please feel free to contact us. We are looking forward to forming successful business relationships with new clients around the world in the near future.
By Aaron from New Zealand - 2018.02.08 16:45
The company leader recept us warmly, through a meticulous and thorough discussion, we signed a purchase order. Hope to cooperate smoothly
By Christina from Poland - 2018.06.05 13:10
