High Quality for Hpv Genotyping Pcr - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test
High Quality for Hpv Genotyping Pcr - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test Detail:
Product name
28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR )
Certificate
CE
Epidemiology
Cervical cancer is one of the most common malignant tumors in the female reproductive tract. The studies have shown that persistent infection and multiple infections of human papillomavirus is one of the important cause of cervical cancer. At present, there is still a lack of recognized effective treatment methods for HPV. Therefore, early detection and early prevention of cervical HPV is the key to blocking canceration. The establishment of a simple, specific and rapid pathogenic diagnostic method is of great significance in the clinical diagnosis of cervical cancer.
Features
● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 25 Copies/reaction
● High specificity: No cross-reactivity with common respiratory pathogens for more accurate results.
● Convenient: One test for multiple results.
Channel
| S/N | Channel | Type |
| PCR-Mix1 | FAM | 16, 18, 31, 56 |
| VIC(HEX) | Internal Control | |
| CY5 | 45, 51, 52, 53 | |
| ROX | 33, 35, 58, 66 | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| CY5 | 40, 42, 43, 82 | |
| ROX | 39, 59, 68, 73 |
PCR Amplification Conditions Setting
| Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
| 1 | 1 cycle | 50℃ | 2 mins | No |
| 2 | 1 cycle | 95℃ | 10 mins | No |
| 3 | 10 cycle | 95℃ | 15 s | No |
| 62℃ | 20 s | No | ||
| 4 | 30 cycle | 95℃ | 15 s | No |
| 58℃ | 30 s | Yes |
Technical Parameters
| Storage | ≤-18℃ in dark |
| Shelf-life | 12 months |
| Specimen Type | Cervical exfoliated cells |
| Ct | ≤28 |
| CV | ≤5.0％ |
| LoD | 25 Copies/reaction |
| Specificity | No cross-reactivity with common reproductive tract pathogens (such as ureaplasma urealyticum, genital tract chlamydia trachomatis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, mold, gardnerella and other HPV types not covered in the kit, etc). |
| Applicable Instruments | It can match the mainstream fluorescent PCR instruments on the market. |
| SLAN-96P Real-Time PCR Systems | |
| ABI 7500 Real-Time PCR Systems | |
| QuantStudio®5 Real-Time PCR Systems | |
| LightCycler®480 Real-Time PCR Systems | |
| LineGene 9600 Plus Real-Time PCR Detection Systems | |
| MA-6000 Real-Time Quantitative Thermal Cycler | |
Main Components
| Catalogue Number | Component (50 tests/kit) | Specification | Quantity | Component Description |
| HWTS-CC003A | HPV WELL1 | 1.3mL/vial | 1 vial | Mixture of specific primers and probes of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66 |
| HPV WELL2 | 1.3mL/vial | 1 vial | Mixture of specific primers and probes of HPV6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83 | |
| HPV Enzyme Mix | 55uL/vial | 1 vial | Mixture of Taq DNA polymerase, Uracil-DNA Glycosylase and glycerin | |
| HPV Positive Control WELL1 | 150μL/vial | 1 vial | Mixture of HPV16, 18, 31, 33, 35, 45, 51, 52, 53, 56, 58, 66 templates and Internal Control template | |
| HPV Positive Control WELL2 | 150μL/vial | 1 vial | Mixture of 6, 11, 26, 39, 40, 42, 43, 44, 54, 59, 61, 68, 73, 81, 82, 83 templates | |
| HPV Blank Control | 300μL/vial | 1 vial | RNase/DNase free H2O |
Total PCR Solution
Option 1.
Option 2.
Product detail pictures:
Related Product Guide:
We now have our individual sales group, layout team, technical team, QC crew and package group. Now we have strict high-quality control procedures for each procedure. Also, all of our workers are experienced in printing discipline for High Quality for Hpv Genotyping Pcr - 28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR ) – Macro & Micro-Test , The product will supply to all over the world, such as: Madrid, Jersey, Austria, We're your reliable partner in the international markets of our products and solutions. We focus on providing service for our clients as a key element in strengthening our long-term relationships. The continual availability of high grade solutions in combination with our excellent pre- and after-sales service ensures strong competitiveness in an increasingly globalized market. We're willing to cooperate with business friends from at home and abroad, to create a great future. Welcome to Visit our factory. Looking forward to have win-win cooperation with you.
By Jack from Serbia - 2018.11.02 11:11
The company can keep up with the changes in this industry market, product updates fast and the price is cheap, this is our second cooperation, it's good.
By James Brown from Paraguay - 2017.12.19 11:10
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