Helicobacter Pylori Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test

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Helicobacter Pylori Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test Detail:

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Helicobacter Pylori Nucleic Acid Detection Kit(Fluorescence PCR)

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Epidemiology

Helicobacter pylori (Hp) is a Gram-negative helical microaerophilic bacterium. Hp has a global infection and is closely related to many upper gastrointestinal diseases. It is an important pathogenic factor for chronic gastritis, gastric ulcer, duodenal ulcer, and upper gastrointestinal tumors, and the World Health Organization has classified it as a class I carcinogen. With the in-depth research, it is found that Hp infection is not only related to gastrointestinal diseases, but also can cause cardiovascular and cerebrovascular diseases, hepatobiliary diseases, chronic bronchitis, iron deficiency anemia and other system diseases, and even induce tumors.

Features

● Multiplex PCR Amplification Technology
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 500Copies/mL
● Convenient: One test for multiple results

Channel

FAM: Helicobacter pylori nucleic acid
VIC (HEX): Internal control

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	2mins	No
2	1 cycle	95℃	5mins	No
3	40 cycles	95℃	10s	No
3	40 cycles	58℃	30s	Yes

Technical Parameters

Storage	≤-18℃ In dark
Shelf-life	12 months
Specimen Type	Human gastric mucosa tissue samples, Saliva
Ct	≤38
CV	≤5.0％
LoD	500Copies/mL

Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.

Main Components

Catalogue Number	Component (50 tests/kit)	Specification	Quantity	Component Description
HWTS-OT075A	Hp Reaction Buffer	1mL/vial	1 vial	Amplification reaction reagents, primer probes, internal control primer probes, Taq enzyme, UDG enzyme, etc.
	Hp Positive Control	600μL/vial	1 vial	Mixture of viral target gene and internal control
	Hp Blank Control	600μL/vial	1 vial	DNase/RNase free H₂O

Total PCR Solution

Helicobacter Pylori Nucleic Acid Detection Kit(Fluorescence PCR)6

Reference

[1] Szczepanik M. Interplay between Helicobacter pylori and the immune system[J]. J Physiol Pharmacol, 2006, 57 ( Suppl 3) : 15- 27.
[2] Huang TC, Lee CL. Diagnosis, Treatment, and Outcome in Patients with Bleeding Peptic Ulcers and Helicobacter pylori Infections[J]. Biomed Res Int, 2014: 658108.
[3] Rajesh Vijayvergiya, Ramalingam Vadivelu. Role of Helicobacter pylori infection in pathogenesis of atherosclerosis[J]. World Journal of Cardiology, 2015, 7(3): 134-143.

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● Gastrointestinal