Helicobacter Pylori Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test
Helicobacter Pylori Nucleic Acid Detection Kit(Fluorescence PCR) – Macro & Micro-Test Detail:
Product name
Helicobacter Pylori Nucleic Acid Detection Kit(Fluorescence PCR)
Certificate
CE
Epidemiology
Helicobacter pylori (Hp) is a Gram-negative helical microaerophilic bacterium. Hp has a global infection and is closely related to many upper gastrointestinal diseases. It is an important pathogenic factor for chronic gastritis, gastric ulcer, duodenal ulcer, and upper gastrointestinal tumors, and the World Health Organization has classified it as a class I carcinogen. With the in-depth research, it is found that Hp infection is not only related to gastrointestinal diseases, but also can cause cardiovascular and cerebrovascular diseases, hepatobiliary diseases, chronic bronchitis, iron deficiency anemia and other system diseases, and even induce tumors.
Features
● Multiplex PCR Amplification Technology
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 500Copies/mL
● Convenient: One test for multiple results
Channel
FAM: Helicobacter pylori nucleic acid
VIC (HEX): Internal control
PCR Amplification Conditions Setting
| Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
| 1 | 1 cycle | 50℃ | 2mins | No |
| 2 | 1 cycle | 95℃ | 5mins | No |
| 3 | 40 cycles | 95℃ | 10s | No |
| 58℃ | 30s | Yes |
Technical Parameters
| Storage | ≤-18℃ In dark |
| Shelf-life | 12 months |
| Specimen Type | Human gastric mucosa tissue samples, Saliva |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 500Copies/mL |
Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.
Main Components
| Catalogue Number | Component (50 tests/kit) | Specification | Quantity | Component Description |
| HWTS-OT075A | Hp Reaction Buffer | 1mL/vial | 1 vial | Amplification reaction reagents, primer probes, internal control primer probes, Taq enzyme, UDG enzyme, etc. |
| Hp Positive Control | 600μL/vial | 1 vial | Mixture of viral target gene and internal control | |
| Hp Blank Control | 600μL/vial | 1 vial | DNase/RNase free H2O |
Total PCR Solution
Reference
[1] Szczepanik M. Interplay between Helicobacter pylori and the immune system[J]. J Physiol Pharmacol, 2006, 57 ( Suppl 3) : 15- 27.
[2] Huang TC, Lee CL. Diagnosis, Treatment, and Outcome in Patients with Bleeding Peptic Ulcers and Helicobacter pylori Infections[J]. Biomed Res Int, 2014: 658108.
[3] Rajesh Vijayvergiya, Ramalingam Vadivelu. Role of Helicobacter pylori infection in pathogenesis of atherosclerosis[J]. World Journal of Cardiology, 2015, 7(3): 134-143.
Product detail pictures:
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