Fast delivery 28 Types Of Hpv Nucleic Acid Detection Kit - Human Papillomavirus (28 Types) Genotyping Detection Kit (Fluorescence PCR) – Macro & Micro-Test
Fast delivery 28 Types Of Hpv Nucleic Acid Detection Kit - Human Papillomavirus (28 Types) Genotyping Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:
Product Name
HWTS-CC004A-Human Papillomavirus (28 Types) Genotyping Detection Kit (Fluorescence PCR)
Epidemiology
The kit uses multiple nucleic acid amplification (PCR) fluorescence detection method. Highly specific primers and probes are designed based on the L1 gene target sequence of HPV. The specific probe is labeled with FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore (HPV35, 44, 45, 54, 56, 68, 82) and ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) at 5’, and the 3’ quencher group is BHQ1 or BHQ2. During the PCR amplification, specific primers and probes bind to their respective target sequences. When Taq enzyme encounters the probes bound to the target sequence, it exerts the function of 5’ end exonuclease to separate the reporter fluorophore from the quencher fluorophore, so that the fluorescence monitoring system can receive the fluorescent signal, that is, every time a DNA strand is amplified, a fluorescent molecule is formed, which realizes the complete synchronization of the accumulation of fluorescent signals and the formation of PCR products, so as to achieve qualitative and genotyping detection of nucleic acids of 28 types of human papillomavirus in cervical exfoliated cell samples.
Channel
| FAM |
16，58，53，73，6，26，40· |
| VIC/HEX |
18，33，51，59，11，81，43 |
| ROX | 31，66，52，39，83，61，42 |
| CY5 | 56，35，45，68，54，44，82 |
Technical Parameters
| Storage | ≤-18℃ In dark |
| Shelf-life |
12 months |
| Specimen Type | Cervical exfoliated cells |
| Ct | ≤25 |
| CV |
≤5.0% |
| LoD |
25 copies/reaction |
| Applicable Instruments | Easy Amp Real-time Fluorescence Isothermal Detection System(HWTS1600)
Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems
SLAN-96P Real-Time PCR Systems
LightCycler®480 Real-Time PCR system
LineGene 9600 Plus Real-Time PCR Detection System
MA-6000 Real-Time Quantitative Thermal Cycler
BioRad CFX96 Real-Time PCR System
BioRad CFX Opus 96 Real-Time PCR System |
Work Flow
Option 1.
Recommended extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).
Option 2.
Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Product detail pictures:
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By Linda from Kenya - 2017.03.28 12:22
This supplier's raw material quality is stable and reliable, has always been in accordance with the requirements of our company to provide the goods that quality meet our requirements.
By Amy from Brasilia - 2018.06.09 12:42
