Factory Free sample Dengue Ns1 Antigen Kit - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test
Factory Free sample Dengue Ns1 Antigen Kit - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test Detail:
Product name
Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)
Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)
Certificate
CE
Epidemiology
Dengue fever (DF), which is induced by denguevirus (DENV) infection, is one of the most epidemic arbovirus infectious diseases. DENV belongs to flavivirus under flaviviridae, and can be classified into 4 serotypes according to surface antigen. Its transmission medium includes Aedes aegypti and Aedes albopictus, prevalent mainly in tropical and subtropical areas.
Clinical manifestations of DENV infection mainly include headache, fever, weakness, enlargement of lymph node, leukopenia and etc., and bleeding, shock, hepatic injury or even death in severe cases. In recent years, climate change, urbanization, quick development of tourism and other factors have provided more rapid and convenient conditions for transmitting and spreading of DF, leading to constant expansion of epidemic area of DF.
Features
● Multiplex PCR Amplification Technology.
● Sub-Package: Safer and easier.
● High sensitivity: 500 Copies/mL.
● High specificity: No cross reactivity with common pathogens causing febrile and hemorrhagic diseases.
Channel
FAM: Dengue Virus I
VIC(HEX): Dengue Virus II
ROX: Dengue Virus III
CY5: Dengue Virus IV
PCR Amplification Conditions Setting
| Step | Cycles | Temperature | Time | Collect Fluorescent Signals or Not |
| 1 | 1 cycle | 50℃ | 30mins | No |
| 2 | 1 cycle | 95℃ | 15mins | No |
| 3 | 40 cycles | 95℃ | 15s | No |
| 60℃ | 45s | Yes |
Technical Parameters
| Storage | |
| Liquid | ≤-18℃ in dark; lyophilization:≤30℃ in dark |
| Shelf-life | 12 months |
| Specimen Type | Fresh Serum |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 500 Copies/mL |
Specificity: Perform cross reaction tests of Japanese encephalitis virus, Forest encephalitis virus, severe fever with thrombocytopenia syndrome, Xinjiang hemorrhagic fever, hantaan virus, hepatitis C virus, influenza A virus, influenza B virus and etc. No cross reaction is detected.
Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
ABI 7500 Fast Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.
BioRad CFX96 Real-Time PCR System.
BioRad CFX Opus 96 Real-Time PCR System.
Main Components
Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)
| Component | Catalogue Number | Quantity | Component Description | |
| HWTS-FE034A | HWTS-FE034B | |||
| Specification(20 tests/kit) | Specification(50 tests/kit) | |||
| DENV Reaction Buffer | 394μL/vial | 985μL/vial | 1 vial | Dengue virus I/II/III/IV primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc. |
| DENV Enzyme Mix | 6μL/vial | 15μL/vial | 1 vial | Reverse transcriptase, etc. |
| DENV Positive Control | 400μL/vial | 1 mL/vial | 1 vial | Mixture of DENV I/II/III/IV templates |
| DENV Blank Control | 400μL/vial | 1 mL/vial | 1 vial | DNase/RNase free H2O |
Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)
| Component | Character | Catalogue Number | Component Description | |
| HWTS-FE004B | HWTS-FE004C | |||
| Specification/Quantity(20 tests/kit) | Specification/Quantity (50 tests/kit) | |||
| DENV Reaction Buffer | lyophilized | 1 bottle | 1 bottle | dengue virus I/II/III/IV primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc. |
| DENV Positive Control | lyophilized | 1 vial | 1 vial | Mixture of DENV I/II/III/IV templates |
| DENV Blank Control | Liquid | 1 vial, 600μL/vial | 1 vial, 600μL/vial | DNase/RNase free H2O |
| Reconstituted Solution | Liquid | 1 vial, 1mL/vial | 1 vial, 1.45mL/vial | DNase/RNase free H2O, etc. |
Total PCR Solution
Reference
[1] Shu P Y, Chang S F, Kuo Y C, et al. Development of Group- and Serotype-Specific One-Step Sybr Green I-Based Real-Time Reverse Transcription-PCR Assay for Dengue Virus[J]. Journal of Clinical Microbiology, 2003, 41(6):2408-2416.
[2] Paudel D, Jarman R , Limkittikul K, et al. Comparison of real-time SYBR green dengue assay with real-time taqman RT-PCR dengue assay and the conventional nested PCR for diagnosis of primary and secondary dengue infection[J]. North American Journal of Medical Sciences, 2011, 3(10):478-485.
[3] Waggoner J J, Ballesteros G, Gresh L, et al. Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection[J]. Journal of Clinical Virology the Official Publication of the Pan American Society for Clinical Virology, 2016:57-61.
[4] Yao J X, Zhao W, Zhang L, et al. Research of Real-Time Fluorescence PCR Detection on Dengue Virus[J]. West China Medical Journal, 2009.
Product detail pictures:
Related Product Guide:
With sophisticated technologies and facilities, strict top quality handle, reasonable value, exceptional support and close co-operation with clients, we are devoted to furnishing the ideal worth for our clients for Factory Free sample Dengue Ns1 Antigen Kit - Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR) – Macro & Micro-Test , The product will supply to all over the world, such as: Liverpool, Egypt, Tajikistan, So that you can utilize the resource from the expanding info in international trade, we welcome shoppers from everywhere on-line and offline. In spite of the good quality solutions we offer, effective and satisfying consultation service is supplied by our specialist after-sale service team. Product lists and detailed parameters and any other info weil be sent to you timely for your inquiries. So please make contact with us by sending us emails or call us if you have any questions about our corporation. ou may also get our address info from our web page and come to our company to get a field survey of our merchandise. We are confident that we are going to share mutual achievement and create strong co-operation relations with our companions in this marketplace. We're searching forward for your inquiries.
By Stephanie from Thailand - 2017.08.16 13:39
The sales manager has a good English level and skilled professional knowledge, we have a good communication. He is a warm and cheerful man, we have a pleasant cooperation and we became very good friends in private.
By Jacqueline from Lebanon - 2018.02.08 16:45
